Fig 1: Immunohistochemical (IHC) staining for semaphorin 5A in gastric cancer tissues.(A) Semaphorin 5A expression in H. pylori-positive gastric cancer tissues. (B) Semaphorin 5A expression in H. pylori-negative gastric cancer tissues. The red arrow indicates positive staining. Scale bars,100 μm. Note: Samples were obtained from 200 patients who underwent surgical resection for gastric cancer with no preoperative chemotherapy or radiotherapy between March 2019 and July 2020 at the Department of Surgery in the First Affiliated Hospital of Kunming Medical University (P.R. China). By observing the status of H. pylori infection, the 200 cases were divided into an infection-positive group and an infection-negative group. All samples underwent Sema5A IHC staining and semiquantitative scoring. Semaphorin 5A expression was associated with H. pylori infection (p < 0.001) (Table 2).